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The
SoloVPE System is the enabling technology behind Slope Spectroscopy
methods. The system is fundametnally a UV-Vis spectroscopy system whose
measurement principles are based on the Beer-Lambert Law. The major
distinction between the SoloVPE and traditional UV-Vis spectrometers is
the ability of the SoloVPE to dynamically vary the measurement
pathlength when traditional methods use a fixed pathlength. The ability
to make measurements at measurements at different pathlengths enables
Slope Spectroscopy methods which relay on Absorbance vs. Pathlength
(Section) data as opposed to traditional spectra. Derived from the
Beer-Lambert Law, the Slope Spectroscopy Equation (m = EC) relates the
slope (m) of the Absorbance vs. Pathlength plot to the concentration (c)
and Extinction Coefficient (E) of the sample being measured. The SoloVPE
Systems ability to make direct measurements of this slope value means
direct measurement of concentration is possible when the Extinction
Coefficient is known. For this reason the SoloVPE System is frequently
referred to as the first Slope Spectrometer.
This capability combined with the ability of the SoloVPE System to make measurements at pathlengths ranging from as small as 0.005 mm up to 15.000 mm makes the system capable of measuring both highly concentrated and very dilute samples. For example, protein sample concentrations as low as 0.01 mg/ml and as high as 300 mg/ml can be measured directly without sample preparation. There are many unique benefits associated with the move to Slope Spectroscopy methods that are saving companies time and money.
To learn more, visit the Resources page, Download the Webinar or Contact Us to Request a Live Webinar or Request a Demonstration.